RNA channelling by the eukaryotic exosome.

نویسندگان

  • Hélène Malet
  • Maya Topf
  • Daniel K Clare
  • Judith Ebert
  • Fabien Bonneau
  • Jerome Basquin
  • Karolina Drazkowska
  • Rafal Tomecki
  • Andrzej Dziembowski
  • Elena Conti
  • Helen R Saibil
  • Esben Lorentzen
چکیده

The eukaryotic exosome is a key nuclease for the degradation, processing and quality control of a wide variety of RNAs. Here, we report electron microscopic reconstructions and pseudo-atomic models of the ten-subunit Saccharomyces cerevisiae exosome in the unbound and RNA-bound states. In the RNA-bound structures, extra density that is visible at the entry and exit sites of the exosome channel indicates that a substrate-threading mechanism is used by the eukaryotic exosome. This channelling mechanism seems to be conserved in exosome-like complexes from all domains of life, and might have been present in the most recent common ancestor.

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عنوان ژورنال:
  • EMBO reports

دوره 11 12  شماره 

صفحات  -

تاریخ انتشار 2010